Ajania Pacifica, a novelty on the horticultural market, is valued both as an ornamental and a medicinal plant. Establishment of numerous breeding programs led to the creation of many cultivars. Therefore, it is important to develop efficient storage methods of the species. The aim of this study was to develop an encapsulation-dehydration cryopreservation protocol of Ajania Pacifica shoot tips.Shoot tips of Ajania ‘Bengo‘were precultured on media with different sucrose and ABA concentrations, encapsulated in 3% sodium alginate and dehydrated osmotically. Subsequently, the beads were desiccated in sterile air flow for various periods and immersed in liquid nitrogen. After thawing the explants were inoculated on various recovery media.Higher (9%) sucrose concentration and addition of ABA (15 μM) during precultured, followed by 4-hour desiccation, as well as, application of cytokines in the post-thawing recovery medium were necessary to provide high survival of Ajania ‘Bengo’ shoot tips.