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Ana Colette Maurício

Universidade do Porto, Portugal

Title: MSCs conditioned media and umbilical cord blood plasma metabolomics and composition: importance for MSCs scale-up expansion for clinical applications

Biography

Biography: Ana Colette Maurício

Abstract

Human mesenchymal stem cells (hMSCs) from umbilical cord blood (UCB) and umbilical cord matrix are tested clinically for a variety of pathologies but in vitro expansion is essential to achieve appropriate cell numbers for clinical use and most applications still need culture media containing fetal bovine serum (FBS). Until today, there is no reliable serum-free medium and the animal sera have several disadvantages. Human umbilical cord blood plasma (hUCBP) can be used as a supplement for hMSCs from the umbilical cord (UC) Wharton’s jelly culture, since UCB is rich in soluble growth factors and due to worldwide increase in the number of cryopreserved UCB units in public and private banks. On the other hand, the culture media enriched in growth factors produced by these hMSCs in expansion (Conditioned medium - CM) can be an alternative to hMSCs application. The CM of the hMSCs from the UC Wharton’s jelly might be a better therapeutic option compared to cell transplantation, as it can benefit from the local tissue response to the secreted molecules without the difficulties and complications associated to the engraftment of the allo- or xeno-transplanted cells. These facts drive us to know the detailed composition of the hUCBP and CM, by 1H-NMR and Multiplexing LASER Bead Technology. hUCBP is an adequate alternative for the FBS and the CM and hUCBP are important sources of growth factors, which can be used in MSCs-based therapies. Some of the major proliferative, chemotactic and immunomodulatory soluble factors (TGF-β, G-CSF, GM-CSF, MCP-1, IL-6, IL-8) were detected in high concentrations in CM and even higher in hUCBP. The results from 1H-NMR spectroscopic analysis of CM endorsed a better understanding of hMSCs metabolism during in vitro culture, and the relative composition of several metabolites present in CM and hUCBP was obtained. The data reinforces the potential use of hUCBP and CM in tissue regeneration and focus the possible use of hUCBP as a substitute for the FBS used in hMSCs cell culture and expansion.