Xiaopu Yuan
Cedars-Sinai Medical Center, USA
Title: Biobanking with histological annotation: A 3000 prostate tissue repository
Biography
Biography: Xiaopu Yuan
Abstract
To provide researchers with the highest quality prostate cancer tissues, we developed a new protocol for tissue collection from prostatectomies. Since prostate cancers cannot be grossly appreciated, microscopy is required to identify tumor areas. We collected 2,737 frozen blocks from 415 consented patients in 2010-2014 under an IRB-approved protocol. During regular submission of radical prostatectomy cases in the clinical pathology laboratory, two central slices of the prostate were obtained for banking. The inked margins were removed and with the rest of the specimen. The remainder was frozen into up to eight cassettes. After the case was signed out, a diagnosis was obtained for all frozen blocks. Two anatomic pathologists reviewed the slides and recorded the tissue size, tumor size and percentage, Gleason sum (GS) and percent inflammation. 451 out of 2,737 blocks contained cancer (16.5%) while 174 out of 415 cases contained cancer (41.9%). The greatest diameter of the tumor ranged from 0.1-0.3 (22%), 0.4-0.9 cm (48%) and ≥1cm (30%). GS 6 was present in 24.8%, GS 7 in 46.1%, GS 8 in 10%, GS 9 in 16%, and GS 10 in 3.1%. Seventy slides contained a tertiary score (15.6%). Inflammation amounted to 10% in 56.1% of slides, 20% in 35.7% of slides and 30-40% in 8.3% of slides. For each slide, histological parameters were entered into designated and searchable fields within the OnCore database. Altogether, our protocol has created a precise inventory in the biobank and significantly increased the speed and efficiency of distributing prostate cancer tissues.