Cryopreservation Methods

The aim of the cryopreservation procedure is to keep away from crystallization and dehydration of the cell. As the freeze factor temperature is approached, ice crystals can form outside of the cell creating an osmotic imbalance with the cell. Excessive dehydration will create nonviable cells. The detrimental effects of dehydration and crystallization can be minimized by doing the following:

  • Controlling the cooling rate 

  • Using cryoprotective agents 

  • Maintaining appropriate storage temperatures

  • Controlling the thawing rate

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